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BIOTECHNOLOGY: PROCESS AND APPLICATION

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BIOTECHNOLOGY: - PROCESS AND APPLICATION Def.-“Biotechnology is a branch of biology which deals with the techniques of using live organisms, enzymes or biological processes to produce products and provides services for human welfare.” The new definition of modern biotechnology given by EFB( The European Federation of Biotechnology) Def-“Biotechnology is the integration of natural science and organisms, cells, parts thereof and molecular analogues for products and services.” “The science which deals with the synthesis of artificial genes, repair of gene, combination of genes from two organisms & manipulation of artificial genes for improvement of living organisms is called genetic engineering”. RECOMBINANT DNA TECHNOLOGY : The idea for recombinant DNA was first proposed by Peter Lobban, a graduate student of Prof. Dale Kaiser in the Biochemistry Department at Stanford University Medical School. The first publications describing the successful production and intracellul...
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Monohybrid cross Andmonohybrid ratio :- Def : A cross between two pure (homozygous) parents having a single pair of contrasting characters is used or studied is called as monohybrid cross, and ratio obtained from monohybrid cross  is called as monohybrid ratio. (Phenotypic ratio = 3:1 & Genotypic ratio = 1;2;1 ) This experiment involving 3 steps - In first step (I) - Mendel selected pure tall pea plant as a female parent and pure Dwarf pea plant as a male parent. He confirmed whether they are true breeding or not by selfing them for three generations. In step second (II) -      Mendel performed artificial cross between female and male parent which were Tall & Dwarf.      Mendel expecting progeny obtained from parent will be intermediate height or 50 % Tall &        50%  dwarf but he surprised and found all were Tall in F 1   generation (Tt).    He started thinking, where...
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